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Updated: May 3, 2026

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Trabecular Meshwork Response to Pressure Elevation in the Living Human Eye

Published on: June 20, 2015

Tissue-based imaging model of human trabecular meshwork.

Edward R Chu¹, Jose M Gonzalez, James C H Tan

¹Department of Ophthalmology, Keck School of Medicine, University of Southern California , Los Angeles, California.

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics

|February 13, 2014

Summary

This summary is machine-generated.

Researchers created a human trabecular meshwork (TM) model using donor tissue. This advanced model enables detailed 3D imaging and analysis of TM cells and extracellular matrix for glaucoma research.

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Area of Science:

Ophthalmology
Biomedical Engineering
Cell Biology

Background:

The trabecular meshwork (TM) is crucial for regulating intraocular pressure.
Understanding TM cellular and extracellular matrix (ECM) biology is vital for glaucoma research.
Existing models often lack the complexity of the native TM environment.

Purpose of the Study:

To develop a viable, tissue-based human TM model for advanced imaging and analysis.
To investigate TM cellularity, ECM composition, and in situ marker expression.
To enable pharmacological studies and 3D quantitative analysis of the TM.

Main Methods:

Utilized viable postmortem corneoscleral donor tissue for the TM model.
Employed two-photon microscopy for deep tissue optical sectioning and imaging.
Combined multimodal imaging techniques: autofluorescence (AF), second harmonic generation (SHG), and epifluorescence.

Main Results:

Demonstrated tissue viability and characterized live TM cells using intravital labeling.
Detailed the structural ECM and localized specific ECM proteins within the TM.
Visualized in situ marker expression, TM actin, inner wall endothelium, and Schlemm's canal.

Conclusions:

The developed human TM model offers a cost-effective platform for studying TM cell biology and pharmacology.
Non-invasive SHG and AF imaging techniques are suitable for clinical applications.
This model facilitates translational research for glaucoma and other TM-related conditions.