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Salting-out thin-layer chromatography and computational analysis of some oral hypoglycemic drugs
Abdel Maaboud Ismail Mohamed1, Fardous Abdel-Fattah Mohamed, Sameh Abdel-Raouf Ahmed
1Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Assiut University, Assiut, 71526, Egypt.
Abstract:
A quantitative structure-retention relationship study of some oral hypoglycemic drugs was carried out using a salting-out thin-layer chromatographic technique. Aqueous solution of ammonium sulfate and acetonitrile was used as a mobile phase. It was established that the applied mobile phase has different effects on retention of the studied oral antidiabetic drugs. The factors that affect the salting-out process were determined. In this study a good correlation between the structures of the investigated drugs with the retention data and molecular descriptors was established throughout computational analysis and using molecular operating environment software, focusing on octanol/water partition coefficient, molar refractivity, total hydrophobic surface area, hydrophobic volume, Van der Waals energy and solvation energy. Quantitative structure-retention relationship modeling for the separation of the investigated drugs was carried out, validated and evaluated.
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