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Partial hepatectomy decreases pancreatic parenchymal enzyme activity
L M Ellis1, D E George, E M Copeland
1Department of Surgery, University of Florida, Gainesville 32611.
The Journal of Surgical Research
|April 1, 1988
Summary
Partial hepatectomy (PH) in rats decreased pancreatic enzyme activity but not pancreatic amylase. Serum amylase levels dropped, indicating a complex response in liver regeneration impacting digestive enzyme function.
Area of Science:
- Gastroenterology
- Hepatology
- Surgical Research
Background:
- Previous studies observed decreased serum amylase after partial hepatectomy (PH).
- The relationship between this serum change and pancreatic exocrine function remained unclear.
Purpose of the Study:
- To investigate if decreased serum amylase post-PH correlates with reduced pancreatic parenchymal exocrine enzyme activity.
- To analyze the impact of PH on pancreatic protein, lipase, and trypsin levels.
Main Methods:
- Male Wistar rats underwent 60% partial hepatectomy (PH), sham laparotomy (SL), or served as controls (CON).
- Animals were pair-fed, and pancreas, blood, and liver were collected after 48 hours for analysis.
- Assays measured serum amylase and pancreatic parenchymal protein, lipase, trypsin, and amylase activity.
Main Results:
- PH significantly reduced pancreatic parenchymal protein (43%), lipase (44%), and trypsin (22%) compared to controls.
- Serum amylase activity decreased by 28% in the PH group, consistent with prior observations.
- Pancreatic parenchymal amylase activity showed no significant change following PH.
Conclusions:
- Partial hepatectomy in rats leads to a decrease in specific pancreatic exocrine enzyme activities (lipase, trypsin) and overall protein content.
- Despite a decrease in serum amylase, pancreatic parenchymal amylase activity is unaffected by PH.
- Further research is needed to determine if these pancreatic functional changes impact digestion and absorption post-liver regeneration.