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Development of an in vitro fibrin clot model to evaluate fibrinolytic agents for wound care application
C Viennet1, C Laurensou2, A C Goydadin3
1PhD, Research Engineer, Engineering and Cutaneous Biology Laboratory, UMR 1098, University of Franche-Comte, France.
Objective:
To describe an in vitro fibrin clot model that could reliably assess the fibrinolytic activity of enzymatic debriding agents for wound care application.
Method:
A model of a fibrin clot was reconstructed in vitro by mixture of human fibrinogen and (alpha)-thrombin supplemented with factor XIII. These clots were then treated with enzymatic ointments. Fibrinolytic activity was investigated by measuring D-dimer levels, using an automated immunoturbidimetric Liatest D-dimer assay.
Results:
Collagenase and papain-urea ointments demonstrated fibrinolytic activity which was macroscopically visible. Their effect was identical on the in vitro reconstructed fibrin clot and ex vivo collected wound fibrin clot; collagenase and papain-urea both induced a complete degradation and dissolution of both fibrin clots after 24 hours of treatment. This was associated with an increase in D-dimer concentration.
Conclusion:
This reconstructed fibrin clot in vitro model has the potential to predict the efficacy of fibrinolytic agents and therefore appears to be a suitable model for in vitro assays.
Declaration Of Interest:
This study was supported by a grant from URGO Laboratory.
