3':5'-cyclic GMP in the yeast Saccharomyces cerevisiae at different metabolic conditions

cGMP is characterized as undetectable in yeast [(1986) Yeast Cell Biology, UCLA Symp. Mol. Cell Biol. (Hicks, J. ed.) p. 495], though in many organisms it contributes specifically to the regulation of metabolism. Here, we detected cGMP, using radioactive labeling and RIA techniques, after extraction of the cells with 1 mol/1 HClO4 at 37 degrees C. The cGMP 0.015-fold cAMP, about 3-times higher with exponentially growing cells than with pressed baker's yeast, and depends on glucose and O2 supply. The PDE inhibitors DMX and IBMX induce in growing cells an additional increase of the cGMP level, without similar effects on cAMP.