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Updated: May 3, 2026
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
Published on: December 23, 2015
Molecularly imprinted cryogels for carbonic anhydrase purification from bovine erythrocyte
Murat Uygun1, A Alev Karagözler, Adil Denizli
1Adnan Menderes University, Koçarlı Vocational and Training School , Aydın , Turkey.
Abstract:
Molecularly imprinted PHEMAH cryogels were synthesized and used for purification of carbonic anhydrase from bovine erythrocyte. Cryogels were prepared with free radical cryopolymerization of 2-hydroxyethyl methacrylate and methacryloylamido histidine and characterized by swelling degree, macroporosity, FTIR, SEM, surface area and elemental analysis. Maximum carbonic anhydrase adsorption of molecularly imprinted PHEMAH cryogel was found to be 3.16 mg/g. Selectivity of the molecularly imprinted cryogel was investigated using albumin, hemoglobin, IgG, γ-globulin, and lysozyme as competitor proteins and selectivity ratios were found to be 15.26, 60.05, 21.88, 17.61, and 17.42, respectively. Carbonic anhydrase purity was demonstrated by SDS-PAGE and zymogram results.
