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Updated: May 3, 2026

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Published on: December 12, 2017
Selective anion sensing by chiral macrocyclic receptors with multiple hydrogen-bonding sites
Tadashi Ema1, Keiichi Okuda, Sagiri Watanabe
1Division of Chemistry and Biotechnology, Graduate School of Natural Science and Technology, Okayama University , Tsushima, Okayama 700-8530, Japan.
Abstract:
Chiral macrocycles featuring sulfonamide and/or amide groups as anion-binding sites were synthesized. X-ray crystal structures and DFT calculations have shown that they adopt different conformations that may lead to unique binding behavior. Indeed, various anions could be sensed by their colorimetric and/or fluorescence signal output. The chiral macrocycles showed chiral recognition for chiral anions. Furthermore, a multisensor array with two or four chiral receptors discriminated seven phosphate anions (AMP, ADP, ATP, CMP, GMP, Pi, and PPi) with 100% classification accuracy.
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