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Related Experiment Videos

pp60c-src expression in the developing rat brain.

C A Cartwright1, R Simantov, W M Cowan

  • 1Molecular Biology and Virology Laboratory, Salk Institute for Biological Studies, San Diego, CA 92138.

Proceedings of the National Academy of Sciences of the United States of America
|May 1, 1988
PubMed
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pp60c-src protein kinase activity is crucial for developing rat brain neurons. Its expression peaks during embryonic development in the striatum and after birth in the hippocampus, supporting neuronal growth and differentiation.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Developmental Biology

Background:

  • pp60c-src is a protein tyrosine kinase implicated in cell growth and differentiation.
  • Understanding its role in the developing brain is essential for comprehending neuronal development and function.

Purpose of the Study:

  • To investigate the developmental expression and activity of pp60c-src in distinct regions of the developing rat brain: striatum, hippocampus, and cerebellum.
  • To correlate pp60c-src activity with key developmental events such as neurogenesis and neuronal growth.

Main Methods:

  • Quantitative analysis of pp60c-src protein kinase activity in tissue homogenates from embryonic and postnatal rat brains.
  • Comparison of kinase activity across different brain regions and developmental time points.

Related Experiment Videos

  • Assessment of pp60c-src protein levels and specific activity.

    • Main Results:

    • Striatal pp60c-src activity peaks at embryonic day 20 (E20), showing a significant increase compared to adult levels and fibroblasts.
    • Hippocampal pp60c-src activity peaks shortly after birth (postnatal day 2, P2) and remains elevated throughout life.
    • Cerebellar pp60c-src activity remains relatively constant from E20 onwards, with levels higher than in fibroblasts.
    • Increased pp60c-src activity in the striatum and hippocampus is attributed to both increased protein amount and enhanced specific activity, coinciding with neurogenesis and neuronal growth periods.

    Conclusions:

    • pp60c-src exhibits distinct developmental expression patterns in different rat brain regions.
    • The observed increase in pp60c-src activity correlates with critical phases of neuronal differentiation and growth.
    • These findings suggest that pp60c-src, a product of a developmentally regulated gene, plays a significant role in neuronal differentiation and/or function.