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Association of plasma soluble α-klotho with pro-endothelin-1 in patients with type 2 diabetes
Jian-Jun Liu1, Sylvia Liu1, Nils G Morgenthaler2
1Clinical Research Unit, Khoo Teck Puat Hospital, Republic of Singapore.
Objectives:
To study the relationship between plasma soluble klotho (sKlotho) and pro-endothelin-1 (proET-1) in patients with type 2 diabetes (T2DM).
Subjects And Methods:
In this cross-sectional study, we recruited 175 T2DM subjects and 56 non-diabetic controls. Plasma sKlotho, proET-1 and extracellular superoxide dismutase (SOD) were measured by ELISA and ILMA, respectively.
Results:
Plasma sKlotho level in patients with T2DM was lower compared to that in non-diabetic controls (416.8±148.1 vs. 494.6±134.3 pg/ml, p=0.001) and showed significant interaction with diabetes status in its association with proET-1. Plasma sKlotho was inversely correlated with proET-1 in T2DM (Rho=-0.410, p<0.0001) but not in non-diabetic controls (Rho=0.091, p=0.505). Multivariable linear regression models revealed that sKlotho was independently associated with proET-1 after adjustment for renal filtration function, albuminuria, diabetes duration, HbA1c, systolic and diastolic blood pressure.
Conclusions:
Plasma sKlotho was associated with proET-1 independent of renal function in patients with T2DM.
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