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Two-dimensional protein mapping by gold stain and immunoblotting
1Institute of Neurology, London, England.
A method is described for mapping individual polypeptides on a two-dimensional separation of a complex mixture of proteins. Polypeptides are identified by immunoblotting on nitrocellulose prestained for total protein by a colloidal gold stain which does not inhibit antibody binding. The same polypeptides can subsequently be identified on gold-stained blots run under identical conditions, without the need for immunoblotting.
A method is described for mapping individual polypeptides on a two-dimensional separation of a complex mixture of proteins. Polypeptides are identified by immunoblotting on nitrocellulose prestained for total protein by a colloidal gold stain which does not inhibit antibody binding. The same polypeptides can subsequently be identified on gold-stained blots run under identical conditions, without the need for immunoblotting.