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Cell type-specific expression of Eps8 in the mouse hippocampus
Chiung-Chun Huang, Yun-Shen Lin, Cheng-Che Lee
1Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. richard@mail.ncku.edu.tw.
BMC Neuroscience
|February 19, 2014
Summary
Epidermal growth factor receptor substrate 8 (Eps8) is found in specific mouse hippocampal interneurons, not pyramidal cells. This suggests Eps8 plays a key role in interneuron development and plasticity.
Area of Science:
- Neuroscience
- Cell Biology
Background:
- Epidermal growth factor receptor substrate 8 (Eps8) regulates actin cytoskeleton dynamics.
- Eps8 is crucial for dendritic spine morphogenesis in cultured hippocampal neurons.
Purpose of the Study:
- To investigate the detailed expression pattern of Eps8 in the mouse hippocampus.
Main Methods:
- Immunohistochemistry was used to detect endogenous Eps8 protein.
- Co-localization studies with cell-type specific markers were performed.
Main Results:
- Eps8 is expressed in neurons (NeuN+) but not glial cells (GFAP+) in the mouse hippocampus CA1.
- Eps8 is predominantly found in GABAergic interneurons (GAD67+) and calbindin-expressing interneurons, but not parvalbumin or calretinin interneurons.
- Eps8 is present in cholecystokinin-expressing interneurons but absent in somatostatin-expressing interneurons.
Conclusions:
- This study reveals a novel cell type-specific expression pattern for Eps8 in the hippocampus.
- Eps8's role in neuronal morphogenesis and structural plasticity may be more significant in interneurons than in pyramidal cells.
