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Related Concept Videos

MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
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Methodological challenges in utilizing miRNAs as circulating biomarkers.

Leni Moldovan1, Kara E Batte, Joanne Trgovcich

  • 1Division of Pulmonary, Allergy, Critical Care, Sleep Medicine, College of Medicine, The Ohio State University, Columbus, OH, USA.

Journal of Cellular and Molecular Medicine
|February 19, 2014
PubMed
Summary
This summary is machine-generated.

Extracellular microRNAs (miRNAs) in blood are promising disease biomarkers. Rigorous methods for isolating and profiling these circulating miRNAs are crucial for accurate diagnostics, particularly in cardiovascular and pulmonary diseases.

Keywords:
biomarkerscirculating microRNAsmicroRNAplasmaprofilingreal-time PCRserum

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key post-transcriptional gene regulators.
  • Extracellular miRNAs found in bodily fluids like blood are implicated as disease biomarkers.
  • Standardized methodologies are needed for reliable biomarker discovery.

Purpose of the Study:

  • To detail critical procedural aspects of miRNA isolation from plasma and serum.
  • To evaluate various profiling methods for quantifying circulating miRNAs.
  • To emphasize methodological rigor for developing diagnostic tools.

Main Methods:

  • Isolation of miRNAs from human plasma and serum samples.
  • Application of different miRNA profiling techniques.
  • Case examples from cardiovascular and pulmonary disease research.

Main Results:

  • Key procedural considerations for successful miRNA isolation were identified.
  • Advantages and disadvantages of various miRNA profiling methods were highlighted.
  • The importance of methodological standardization was demonstrated.

Conclusions:

  • Circulating miRNAs hold significant potential as diagnostic biomarkers.
  • Strict adherence to methodological protocols is essential for reliable miRNA-based diagnostics.
  • Further research focusing on standardization will advance the clinical application of miRNAs.