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Preparation of a High-quality Primary Cell Culture from Fish Pituitaries
Published on: August 28, 2018
Transcriptional changes caused by bisphenol A in Oryzias javanicus, a fish species highly adaptable to environmental
Seonock Woo1, Vianney Denis2, Seungshic Yum3
1South Sea Environment Research Division, Korea Institute of Ocean Science and Technology, Geoje 656-830, Korea. cwoo@kiost.ac.
Abstract:
The Javanese medaka, Oryzias javanicus, is a fish highly adaptable to various environmental salinities. Here, we investigated the effects of the environmental pollutant bisphenol A (BPA; an endocrine disrupting chemical) on gene expression levels in this species acclimated to different salinities. Using cDNA microarrays, we detected the induction of differential expression of genes by BPA, and compared the transcriptional changes caused by chemical exposure at different salinities. There were marked transcriptional changes induced by BPA between treatments. While 533 genes were induced by a factor of more than two when O. javanicus was exposed to BPA in seawater, only 215 genes were induced in freshwater. Among those genes, only 78 were shared and changed significantly their expression in both seawater and freshwater. Those genes were mainly involved in cellular processes and signaling pathway. We then categorized by functional group genes specifically induced by BPA exposure in seawater or freshwater. Gene expression changes were further confirmed in O. javanicus exposed to various concentrations of BPA, using quantitative real-time reverse transcription PCR based on primer sets for 28 selected genes.
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