Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Veratridine modifies open sodium channels.

S Barnes1, B Hille

  • 1Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195.

The Journal of General Physiology
|March 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imagining Futures: Evaluation of a blended programme of dialectical behaviour therapy and the creative arts for young women with a history of self-harm.

The British journal of clinical psychology·2025
Same author

Disseminated tuberculosis, CMV viraemia & haemophagocytic-lymphohistiocystosis syndrome in an adult patient with anti- IFNγ autoantibodies - case report and brief review.

Clinical immunology communications·2023
Same author

Associations between oral health and general health: a surveywide association study of the NHANES.

Community dental health·2021
Same author

Multicentre evaluation of Erytra Eflexis®, a benchtop fully automated analyser with a compact design for routine use in blood transfusion laboratory.

Transfusion medicine (Oxford, England)·2019
Same author

Pathogenicity of the bacterium New Zealand rickettsia-like organism (NZ-RLO2) in Chinook salmon Oncorhynchus tshawytscha smolt.

Diseases of aquatic organisms·2019
Same author

Validity of an ultra-wideband local positioning system to measure locomotion in indoor sports.

Journal of sports sciences·2017
Same journal

Conformational changes upon pore blocker removal reveal conductive states of TMEM16A.

The Journal of general physiology·2026
Same journal

On the mechanism of hypomagnesemia with treatment-resistant seizures caused by variants of the Na+,K+-ATPase α1 subunit (ATP1A1).

The Journal of general physiology·2026
Same journal

Label-free real-time imaging of mitochondrial matrix volume changes and permeability transition in living cells.

The Journal of general physiology·2026
Same journal

Differential regulation of β1-dependent voltage shifts and kinetic modulation by an extracellular glutamate in NaV1.6 VSDIV.

The Journal of general physiology·2026
Same journal

Mechanistic insights into DCPIB inhibition of VRAC: Electrostatic control and binding plasticity.

The Journal of general physiology·2026
Same journal

An epilepsy-associated KV3.1 potassium channel variant acts via dominant-positive effect.

The Journal of general physiology·2026
See all related articles

Veratridine neurotoxin modification of sodium (Na) channels requires channels to be open. This state-dependent modification, observed in neuroblastoma cells, alters channel properties like conductance and open duration.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biophysics

Background:

  • Sodium channels are crucial for neuronal function.
  • Neurotoxins can alter ion channel behavior.
  • Veratridine is an alkaloid neurotoxin known to affect Na channels.

Purpose of the Study:

  • To investigate the state dependence of Na channel modification by veratridine.
  • To determine if Na channel opening is a prerequisite for veratridine modification.
  • To characterize the biophysical properties of veratridine-modified Na channels.

Main Methods:

  • Single-channel and whole-cell voltage-clamp recordings in neuroblastoma cells.
  • Application of veratridine and depolarizing voltage pulses.
  • Analysis of Na current amplitude, channel kinetics, and conductance.

Related Experiment Videos

Main Results:

  • Veratridine modification of Na channels is state-dependent, requiring channels to be in the open state.
  • Modification reduces Na channel current amplitude and increases burst duration.
  • Modified channels exhibit a significantly lower slope conductance (4 pS) compared to normal channels.
  • Modification is use-dependent and linked to depolarizing pulses and prior channel opening.

Conclusions:

  • Na channel modification by veratridine occurs from the open state.
  • Veratridine binding alters the biophysical properties of Na channels.
  • This study provides insights into the mechanism of neurotoxin-induced ion channel modulation.