Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

993
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
993
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

870
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
870

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two- and three-dimensional electron imaging of beam-sensitive specimens.

Micron (Oxford, England : 1993)·2025
Same author

Voxel dose-limited resolution for thick beam-sensitive specimens imaged in a TEM or STEM.

Micron (Oxford, England : 1993)·2023
Same author

Transmission electron microscopy of thick polymer and biological specimens.

Micron (Oxford, England : 1993)·2023
Same author

Spatial resolution in transmission electron microscopy.

Micron (Oxford, England : 1993)·2022
Same author

Spatial resolution in secondary-electron microscopy.

Microscopy (Oxford, England)·2022
Same author

Direct measurement of the PSF for Coulomb delocalization - a reconsideration.

Ultramicroscopy·2021

Related Experiment Video

Updated: May 2, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.0K

Prospects for vibrational-mode EELS with high spatial resolution.

R F Egerton1

  • 1Physics Department,University of Alberta,Edmonton,Canada T6G 2E1.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|February 20, 2014
PubMed
Summary
This summary is machine-generated.

Measuring vibrational modes using electron energy loss spectroscopy is limited by the zero-loss peak

More Related Videos

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

20.8K
Spectral Reflectometric Microscopy on Myelinated Axons In Situ
09:13

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Published on: July 2, 2018

6.8K

Related Experiment Videos

Last Updated: May 2, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

7.0K
Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid
10:25

Sub-nanometer Resolution Imaging with Amplitude-modulation Atomic Force Microscopy in Liquid

Published on: December 20, 2016

20.8K
Spectral Reflectometric Microscopy on Myelinated Axons In Situ
09:13

Spectral Reflectometric Microscopy on Myelinated Axons In Situ

Published on: July 2, 2018

6.8K

Area of Science:

  • Materials Science
  • Spectroscopy
  • Electron Microscopy

Background:

  • Transmission electron microscopy (TEM) is a powerful tool for materials analysis.
  • Electron energy loss spectroscopy (EELS) provides information on electronic and vibrational properties.
  • Previous work by Geiger and co-workers established foundational measurements.

Purpose of the Study:

  • To investigate the feasibility and challenges of measuring vibrational energy loss modes in TEM.
  • To identify limitations in current high-resolution EELS techniques for vibrational analysis.
  • To assess the applicability of this method for organic specimens.

Main Methods:

  • Utilizing a transmission electron microscope equipped with a monochromator.
  • Employing a high-resolution energy-loss spectrometer for spectral acquisition.
  • Analyzing spectral data, focusing on the zero-loss peak characteristics.

Main Results:

  • The tail of the zero-loss peak, not its full-width at half-maximum, presents a significant limitation.
  • Low oscillator strengths and small cross-sections are inherent challenges.
  • Potential for radiation damage in organic specimens restricts spatial resolution.

Conclusions:

  • The tail of the zero-loss peak is a critical factor affecting the measurement of vibrational modes.
  • Radiation damage and scattering delocalization pose limitations for organic materials.
  • Further optimization is needed for high-resolution vibrational EELS.