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Updated: May 2, 2026

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
Published on: February 12, 2022
FRET-based optical assay for selection of artificial riboswitches
Svetlana V Harbaugh1, Molly E Chapleau, Yaroslav G Chushak
1The Henry M. Jackson Foundation, Bethesda, MD, USA.
Abstract:
Artificial riboswitches are engineered to regulate gene expression in response to a variety of non-endogenous small molecules and, therefore, can be useful tools to reprogram cellular behavior for different applications. A new synthetic riboswitch can be created by linking an in vitro-selected aptamer with a randomized expression platform followed by in vivo selection and screening. Here, we describe an in vivo selection and screening technique to discover artificial riboswitches in E. coli cells that is based on TEV protease-FRET substrate reporter system.
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