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Updated: May 2, 2026

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
Published on: June 27, 2012
In vitro assay of pre-mRNA splicing in mammalian nuclear extract
Maliheh Movassat1, William F Mueller, Klemens J Hertel
1Department of Microbiology & Molecular Genetics, University of California, Irvine, CA, USA.
Abstract:
The in vitro splicing assay is a valuable technique that can be used to study the mechanism and machinery involved in the splicing process. The ability to investigate various aspects of splicing and alternative splicing appears to be endless due to the flexibility of this assay. Here, we describe the tools and techniques necessary to carry out an in vitro splicing assay. Through the use of radiolabeled pre-mRNA and crude nuclear extract, spliced mRNAs can be purified and visualized by autoradiography for downstream analysis.
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