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Updated: May 2, 2026
Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface
Published on: May 21, 2015
Multivariate Analysis Using High Definition Flow Cytometry Reveals Distinct T Cell Repertoires between the
Michelle A Neller1, Brigitte Santner-Nanan2, Rebekah M Brennan1
1Human Immunity Laboratory and Cellular Immunology Laboratory, QIMR Berghofer Medical Research Institute , Brisbane, QLD , Australia.
Abstract:
The human T cell compartment is a complex system and while some information is known on repertoire composition and dynamics in the peripheral blood, little is known about repertoire composition at different anatomical sites. Here, we determine the T cell receptor beta variable (TRBV) repertoire at the decidua and compare it with the peripheral blood during normal pregnancy and pre-eclampsia. We found total T cell subset disparity of up to 58% between sites, including large signature TRBV expansions unique to the fetal-maternal interface. Defining the functional nature and specificity of compartment-specific T cells will be necessary if we are to understand localized immunity, tolerance, and pathogenesis.
