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Functional expression study of igf2 antisense transcript in mouse.

Carolina Duart-Garcia1, Martin H Braunschweig2

  • 1Institute of Genetics, Vetsuisse Faculty, University of Bern, Bremgartenstrasse 109a, 3001 Berne, Switzerland ; Graduate School for Cellular and Biomedical Sciences, Theodor Kocher Institute, University of Bern, Freiestrasse 1, 3012 Berne, Switzerland.

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|February 20, 2014
PubMed
Summary
This summary is machine-generated.

The insulin-like growth factor antisense gene (Igf2as) is expressed in early mouse development and placenta. This study found Igf2as does not regulate Igf2, suggesting a reevaluation of the ΔDMR1-U2 knockout mouse model phenotype.

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Area of Science:

  • Developmental biology
  • Genetics
  • Molecular biology

Background:

  • The role of the insulin-like growth factor antisense gene (Igf2as) in mammalian development is not fully understood.
  • Previous studies linked intrauterine growth restriction in a specific mouse model to Igf2 transcription, but the involvement of Igf2as was unclear.

Purpose of the Study:

  • To investigate the expression patterns of Igf2as in various mouse tissues during development.
  • To determine if Igf2as interacts with or regulates Igf2 transcripts.
  • To reassess the phenotype of the ΔDMR1-U2 knockout mouse model.

Main Methods:

  • Analysis of Igf2as gene expression in different mouse tissues (fetal, newborn, adult liver, muscle, placenta, brain).
  • Investigation of Igf2as expression in differentiating C2C12 cells.
  • Examination of Igf2 expression in ΔDMR1-U2 knockout mouse placentas lacking Igf2as transcription.

Main Results:

  • Igf2as expression was high in fetal and newborn liver and muscle, and also detected in placenta and brain.
  • No consistent evidence of interaction between Igf2 and Igf2as transcripts was found.
  • Igf2 expression in knockout placentas lacking Igf2as was comparable to wild-type, indicating Igf2as does not regulate Igf2.

Conclusions:

  • Igf2as plays a role in early mouse development and placental function.
  • Igf2as does not regulate Igf2 sense transcripts.
  • The phenotype of the ΔDMR1-U2 knockout mouse should be reconsidered due to the functional Igf2as gene.