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Updated: May 2, 2026
Indel Detection following CRISPR/Cas9 Mutagenesis using High-resolution Melt Analysis in the Mosquito Aedes aegypti
Published on: September 10, 2021
Differentiation of Aedes atlanticus and Aedes tormentor by restriction fragment length polymorphisms of the second
Charles B Sither1, Virginia E Hopkins1, Bruce A Harrison1
1Vector-Borne Infectious Disease Laboratory, Environmental Health Sciences Program, College of Health and Human Sciences, Western Carolina University, Cullowhee, NC 28723, USA.
Abstract:
Using novel DNA sequence data, we designed a restriction enzyme assay that distinguishes Aedes atlanticus and Ae. tormentor, based on size polymorphisms. The restriction endonuclease Hpy188I digests polymerase chain reaction-amplified 2nd internal transcribed spacer products once for Ae. atlanticus and twice for Ae. tormentor, thus providing a useful method for identifying adult female collections that are generally considered morphologically indistinguishable.
