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Updated: May 2, 2026

High-resolution Time-lapse Imaging and Automated Analysis of Microtubule Dynamics in Living Human Umbilical Vein Endothelial Cells
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CYLD coordinates with EB1 to regulate microtubule dynamics and cell migration.

Dengwen Li1, Jinmin Gao1, Yunfan Yang1

  • 1State Key Laboratory of Medicinal Chemical Biology; College of Life Sciences; Nankai University; Tianjin, China.

Cell Cycle (Georgetown, Tex.)
|February 21, 2014
PubMed
Summary

Cylindromatosis (CYLD) protein interacts with EB1 to regulate microtubule dynamics and cell migration. This discovery provides new insights into cellular movement and microtubule-associated processes.

Keywords:
CYLDEB1cell migrationcentrosomemicrotubuleyeast-two hybrid

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cylindromatosis (CYLD) is a deubiquitinase known to modulate cell signaling in inflammation and tumorigenesis.
  • CYLD regulates microtubule dynamics and stimulates cell migration, but the underlying mechanisms remain unclear.

Purpose of the Study:

  • To investigate the molecular mechanisms connecting CYLD's regulation of microtubule dynamics to its role in cell migration.
  • To identify proteins that interact with CYLD in the context of cell migration.

Main Methods:

  • Yeast two-hybrid screening was employed to identify CYLD-interacting proteins.
  • Co-immunoprecipitation and in vitro assays were used to confirm interactions and colocalization.
  • Microscopy and in vitro microtubule assembly assays were performed.

Main Results:

  • End-binding protein 1 (EB1) was identified as a CYLD-interacting protein.
  • CYLD and EB1 colocalize at microtubule plus ends and their association increases during cell migration.
  • CYLD and EB1 cooperate to regulate tail retraction, centrosome reorientation, and microtubule stabilization in migrating cells.
  • CYLD and EB1 coordinate microtubule assembly, nucleation, and growth.

Conclusions:

  • CYLD interacts with EB1 to orchestrate microtubule dynamics essential for cell migration.
  • This study elucidates the mechanistic link between CYLD, microtubule regulation, and cell motility.
  • Coordinated actions of microtubule-binding proteins are crucial for cellular events mediated by microtubules.