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Updated: May 2, 2026

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

Published on: February 2, 2016

Triplex-mediated genome targeting and editing.

Faisal Reza¹, Peter M Glazer

¹Departments of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 22, 2014

Summary

This summary is machine-generated.

Triplex-forming molecules like oligonucleotides and peptide nucleic acids enable precise genome editing. This approach facilitates high-fidelity gene modification, repair, and regulation for therapeutic applications.

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Last Updated: May 2, 2026

Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation

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Published on: February 2, 2016

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Published on: March 12, 2018

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Genome editing relies on introducing molecules to induce endogenous recombination.
Sequence-specific binding molecules, such as triplex-forming oligonucleotides (TFOs) and peptide nucleic acids (PNAs), are key tools.

Purpose of the Study:

To review the state-of-the-art in triplex-mediated genome targeting and editing.
To explore potential translational and therapeutic applications of this technology.

Main Methods:

Design, delivery, and application of triplex-forming and recombinagenic molecules.
Identification of endogenous genome targeting and editing sites.
Techniques for assessing targeting and editing efficiency.

Main Results:

Triplex-mediated genome editing offers high-fidelity and low off-target mutagenesis.
This approach enables precise genome repair, expression modulation, and regulation.
Methods for safe, heritable genomic modifications are detailed.

Conclusions:

Triplex-mediated genome editing is a powerful strategy for precise genetic modifications.
The technology holds significant promise for future translational and therapeutic applications in medicine.