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[Study on a collagenase protocol to extract DNA from remnant feathers in edible bird's nest]
Ling-Li Wang1, Nian Chen1, Wei-Wei Zhang1
1School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Objective:
To establish a method for extracting genomic DNA from rudimental bird feather from the precious edible bird's nest (EBN) harvested from the swiftlet cave.
Methods:
Observed the EBN using endoscopic and studied the influence of adding collagenase on the extracting yield of DNA. PCR amplification and sequencing for the extraction was also conducted.
Results:
Collagenase was used in addition to protease K which could substantively increase the DNA yield. The DNA extracted by this method could be used for PCR and other molecular biology analyses.
Conclusion:
This method can be applied to identify the species types in biological products, especially for animal tissue materials that rich in collagen.
