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[AFLP analysis of three species from Akebia genus]
Pei-Bei Huang1, Shi-Hua Chen2, Fei Wang2
1Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China. hhhpei@tom.com
Objective:
To establish the DNA fingerprints of three species from Akebia genus markers and find the feasibility in analyzing their genetic diversity, relationship and germplasm identification by Amplified Fragment Length Polymorphsm (AFLP).
Methods:
The DNA polymorphism of 12 samples from three species of Akebia genus were detected by AFLP molecular markers.
Results:
Among 64 primer pairs,8 were found to yield rich band patterns and high polymorphism. After amplification with these 8 primer pairs to 12 samples, a total of 977 polymorphic DNA bands were detected. The polymorphism rate reached 92.61%. The coefficient of genetic similarity and UPGMA clustering analysis were consistent with the classification based on morphological observations. The coefficient of genetic similarity was 0.49 and the 12 samples could be clustered into 2 groups. Akebia quinata samples formed a single cluster and Akebia trifoliata var. australis and Akebia trifoliata samples formed another cluster.
Conclusion:
AFLP markers can be potentially used in analyzing of genetic diversity, relationship and germplasm identification of these medicinal plants.
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