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Related Experiment Videos

Phase partitioning in space and on earth.

J M Van Alstine1, L J Karr, J M Harris

  • 1Biophysics Branch, NASA/Marshall Space Flight Center, Huntsville, AL 35812.

Advances in Experimental Medicine and Biology
|January 1, 1987
PubMed
Summary

Polymer-based aqueous two-phase systems enable differential partitioning of biological materials. This technique is being explored for low-gravity biomaterials processing, leveraging unique demixing properties in space.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Biophysics

Background:

  • Neutral polymers like dextran and poly(ethylene glycol) (PEG) form aqueous two-phase systems (ATPS).
  • Biological particles and macromolecules partition differentially between these phases and the interface.
  • Partitioning behavior relates to surface properties like membrane charge and lipid composition.

Purpose of the Study:

  • Investigate the influence of gravity on ATPS separation efficiency.
  • Explore the potential of ATPS for low-gravity biomaterials processing.
  • Understand polymer surface coating effects on phase separation and electroosmosis.

Main Methods:

  • Utilizing dextran-poly(ethylene glycol) aqueous two-phase systems.
  • Studying demixing phenomena in Earth and low-gravity environments.

Related Experiment Videos

  • Synthesizing PEG-derivatized antibodies for immunoaffinity partitioning.
  • Investigating polymer surface coatings for phase localization control.

    • Main Results:

    • Biological particles and macromolecules exhibit differential partitioning in ATPS.
    • Low-gravity conditions alter demixing primarily through coalescence.
    • Polymer surface coatings influence phase localization and electroosmosis.
    • PEG-derivatized antibodies facilitate specific cell partitioning.

    Conclusions:

    • Aqueous two-phase systems offer versatile bioseparation capabilities.
    • Understanding gravity's influence is crucial for space-based biomaterials processing.
    • ATPS, particularly with affinity ligands, holds promise for advanced bioparticle separation in microgravity.