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Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Related Experiment Video

Updated: May 2, 2026

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
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Plasmon-based tomographic microscopy.

Juan Elezgaray, Lotfi Berguiga, Françoise Argoul

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    Summary
    This summary is machine-generated.

    Scanning surface plasmon microscopy (SSPM) uses tomographic techniques for super-resolution imaging of dielectric objects. This advanced microscopy offers higher contrast and reduced noise compared to other far-field methods.

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    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Microscopy

    Background:

    • Surface plasmons exhibit high sensitivity to changes near dielectric-metal interfaces.
    • Conventional microscopy is limited by diffraction, restricting resolution.
    • Scanning surface plasmon microscopy (SSPM) leverages these plasmonic properties for imaging.

    Purpose of the Study:

    • To demonstrate the application of tomographic techniques in SSPM.
    • To achieve resolutions beyond the diffraction limit using SSPM.
    • To evaluate the noise sensitivity and contrast ratio of SSPM.

    Main Methods:

    • Applying tomographic reconstruction methods to SSPM data.
    • Imaging dielectric objects with the scanning surface plasmon microscope.
    • Analyzing the resolution limits and noise performance.

    Main Results:

    • Tomographic techniques enable SSPM to surpass the diffraction-limited resolution.
    • High resolution is achievable even outside the multiple scattering regime.
    • SSPM demonstrates a higher contrast ratio, leading to reduced noise sensitivity.

    Conclusions:

    • SSPM, enhanced with tomography, provides super-resolution imaging capabilities.
    • The technique offers superior resolution and contrast compared to conventional far-field microscopies.
    • SSPM presents a promising tool for high-resolution imaging with enhanced noise immunity.