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Harmine as a substitute for 33258 Hoechst in the FPG technique
M G Gutiérrez-Gonzálvez1, M J Hazen, R H Espelosín
1Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Histochemistry
|January 1, 1988
Abstract:
We studied the effectiveness of harmine as a substitute for 33258 Hoechst in the fluorescence-plus-Giemsa technique, using Allium cepa chromosomes after 5-bromo-2'-deoxyuridine (BrdU) incorporation. Harmine showed a photosensitizing capacity which was somewhat higher than 33258 Hoechst and used half of the time established for the usual treatment.