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[Combined effects of T-2 toxin and selenium deficiency on lipid peroxidation in rat's brain]
Ruijuan Zhang1, Dandan Xu1, Zhilun Wang1
1School of Public Health of Xi'an Jiaotong University Health Science Center, Nutrition and Food Safety Engineering Research Center of Shaanxi Province, Key Laboratory of Environment and Genes Related to Diseases (Xi' an Jiaotong University), Ministry of Education, Xi' an, Shaanxi 710061, China.
Objective:
To explore the effects of T-2 toxin and it's synergy with low selenium on lipid peroxidation in rats' brain.
Methods:
Early weaning male SD (Sprague-Dawley) rats were randomly divided into normal group and low-selenium model group by weight after 1 week for adaptive feeding, and respectively fed normal diet and low-selenium diet. After the low Se model was succeed, the normal group were randomly divided into control group, low T-2 group, high T-2 group, the model group were randomly divided into low Se group, low Se + low T-2 group, low Se + high T-2 group. Then give T-2 toxin to the low T-2 group, low Se + low T-2 group at the dose of 0.1 mg/(kg x d), and high T-2 group, low Se + high T-2 group at the dose of 0.2 (kg x d), intragastric administration for 4 weeks. We take the brain sample, observed the morphological changes of brain tissue after HE staining, measured the content of MDA and the activity of GSH-Px to observe the T-2 toxin' effect of antioxidant on rat brain.
Results:
The activity of rats' brain GSH-Px in all low Se groups were lower than the control group (P < 0.05). The content of rats' brain MDA in all experimental group were higher than the control group (P < 0.05). In all experimental group, the structure, the neurons, the nuclei of rats' brain tissue have varying degrees of damage and destruction.
Conclusion:
The single factor of T-2 toxin can cause lipid peroxidation in brain, lower the activity of GSH-Px and higher the lever of MDA. The single factor of low Se can induce lipid peroxidation in rats' brain, lower the activity of GSH-Px, higher the level of MDA, With the condition of low Se, the effect of T-2 toxin on lipid peroxidation in rats' brain was more obviously than the single factor. There have synergistic effect T-2 toxin with low Se.
