Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Double-staining methods at the ultrastructural level applying colloidal gold conjugates.

R Holm1, J M Nesland, A Attramadal

  • 1Department of Pathology, Norwegian Radium Hospital, Oslo.

Ultrastructural Pathology
|May 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modification of extracorporeal photopheresis technology with porphyrin precursors. Comparison between 8-methoxypsoralen and hexaminolevulinate in killing human T-cell lymphoma cell lines in vitro.

Biochimica et biophysica acta·2014
Same author

Identifying microRNAs regulating B7-H3 in breast cancer: the clinical impact of microRNA-29c.

British journal of cancer·2014
Same author

Malignant ovarian germ cell tumors: presentation, survival and second cancer in a population based Norwegian cohort (1953-2009).

Gynecologic oncology·2013
Same author

Disseminated tumour cells in the bone marrow in early breast cancer: morphological categories of immunocytochemically positive cells have different impact on clinical outcome.

Breast cancer research and treatment·2013
Same author

EMMPRIN is associated with S100A4 and predicts patient outcome in colorectal cancer.

British journal of cancer·2012
Same author

Fine needle aspirations in oncology.

Arkhiv patologii·2011
Same journal

Autism spectrum disorder (ASD): zinc suppresses cerebellar ultrastructural alterations and modulates the oxidative stress/gliosis/neuronal loss axis-mediated ASD.

Ultrastructural pathology·2026
Same journal

Alteration of adult neural stem cells by gold nanoparticles in a demyelination model induced in the hippocampus of adult male mice: a structural and ultrastructural study.

Ultrastructural pathology·2026
Same journal

The potential neuroprotective effect of apigenin on cerebellar cortex alterations in valproic acid-induced autism model of male albino rat pups: a histological and immunohistochemical study.

Ultrastructural pathology·2026
Same journal

Deposits of C3 glomerulopathies have ultrastructural hints to correlate with immunofluorescent findings.

Ultrastructural pathology·2026
Same journal

Effect of concurrent administration of granulocyte colony stimulating factor and pirfenidone on experimentally induced lung fibrosis in adult male albino rats (a histological, immunohistochemical & ultrastructural study).

Ultrastructural pathology·2026
Same journal

From lamellar and storage bodies to mitophagy: a comprehensive view of mitochondrial ultrastructure in differentiated SH-SY5Y cells.

Ultrastructural pathology·2026
See all related articles

Four of six double immunogold staining methods yielded satisfactory results. Two methods showed cross-reactivity, indicating limitations in sequential or amplified formaldehyde blockade techniques for reliable double labeling.

Area of Science:

  • Immunoelectron microscopy
  • Biotechnology

Background:

  • Double gold-labeling techniques are crucial for visualizing multiple antigens simultaneously in electron microscopy.
  • Evaluating the efficacy and specificity of different double-staining protocols is essential for accurate ultrastructural analysis.

Purpose of the Study:

  • To review existing double gold-labeling methods.
  • To evaluate the performance of six distinct double-staining techniques using indirect immunogold and protein A-gold approaches.
  • To identify reliable methods for double immunolabeling in electron microscopy.

Main Methods:

  • Review of established double gold-labeling protocols.
  • Application and assessment of six double-staining methods, including indirect double immunogold staining, protein A-gold staining (two-face, amplified, and formaldehyde blockade variants), and sequential methods.

Related Experiment Videos

  • Analysis of cross-reactivity between sequential staining steps.

    • Main Results:

    • Four double-staining methods demonstrated satisfactory results: indirect double immunogold staining, two-face protein A-gold staining, two-face amplified protein A-gold staining, and formaldehyde blockade protein A-gold staining.
    • The sequential double protein A-gold staining and formaldehyde blockade amplified protein A-gold staining methods exhibited undesirable cross-reactions between the primary and secondary labeling steps.

    Conclusions:

    • The indirect double immunogold staining, two-face protein A-gold staining, two-face amplified protein A-gold staining, and formaldehyde blockade protein A-gold staining methods are reliable for double labeling.
    • Sequential and amplified formaldehyde blockade protein A-gold staining methods are not recommended due to cross-reactivity issues.