Double-staining methods at the ultrastructural level applying colloidal gold conjugates
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R Holm1, J M Nesland, A Attramadal
1Department of Pathology, Norwegian Radium Hospital, Oslo.
Ultrastructural Pathology
|May 1, 1988
Abstract:
Several double gold-labeling methods have been reviewed, and our own results of applying some of these methods have been included. Of the six double-staining methods tested, four gave satisfactory results (indirect double immunogold staining method, two-face protein A-gold staining method, two-face amplified protein A-gold staining method, and formaldehyde blockade protein A-gold staining method). The other two techniques (sequential double protein A-gold staining method and formaldehyde blockade amplified protein A-gold staining method) showed cross-reaction between the first and second staining sequence.