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Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...

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Updated: May 2, 2026

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

Caspase-2 protocols.

Loretta Dorstyn¹, Sharad Kumar

¹Centre for Cancer Biology, SA Pathology, Adelaide, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|February 26, 2014

Summary

This summary is machine-generated.

Caspase-2, a key protein in apoptosis, has unique nuclear functions. This study details methods to measure caspase-2 activity, aiding research into its roles in cell death, tumor suppression, and aging.

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Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

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Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

Area of Science:

Molecular Biology
Cell Biology
Biochemistry

Background:

Caspase-2 plays roles in apoptosis, tumor suppression, and aging.
It uniquely localizes to the nucleus, but its function there is unclear.
Caspase-2 activation involves homodimerization and proteolytic processing during apoptosis signaling.

Purpose of the Study:

To outline methods for purifying recombinant caspase-2.
To describe techniques for assessing caspase-2 activity and function.
To facilitate research on caspase-2's diverse cellular roles.

Main Methods:

Purification of recombinant caspase-2.
Assessing caspase-2 dimerization and zymogen cleavage.
Measuring cleavage of caspase-2 substrates.
In vitro, cell culture, and tissue extract assays.

Main Results:

Established protocols for caspase-2 purification.
Validated methods for measuring caspase-2 activation.
Provided tools to study caspase-2 function in various biological contexts.

Conclusions:

Standardized methods are crucial for studying caspase-2.
Understanding caspase-2 activity is key to elucidating its roles in apoptosis and non-apoptotic pathways.
These methods will advance research into caspase-2's involvement in diseases like cancer and aging.