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Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...

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Related Experiment Video

Updated: May 2, 2026

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

Caspase protocols in mice.

Varsha Kaushal¹, Christian Herzog, Randy S Haun

¹Biology Department, Hendrix College, Conway, AR, USA.

Methods in Molecular Biology (Clifton, N.J.)

|February 26, 2014

Summary

This summary is machine-generated.

Detecting caspase activation in mouse tissues involves multiple methods. Researchers use substrate cleavage, western blots, and immunostaining to confirm caspase activity in basic and clinical studies.

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Last Updated: May 2, 2026

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

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Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

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Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

Area of Science:

Biochemistry
Cell Biology
Molecular Biology

Background:

Caspases are critical cysteine proteases executing apoptosis.
Numerous methods exist to detect caspase activation in biological samples.
Caspase activity is vital in both fundamental research and clinical applications.

Purpose of the Study:

To review and summarize methods for detecting caspase activation in mouse tissues.
To highlight the importance of using multiple techniques for reliable caspase detection.

Main Methods:

Monitoring caspase activation via cleavage of specific synthetic substrates.
Western blot analysis to detect cleaved caspases in tissue extracts.
Immunostaining of tissue sections using antibodies against active caspases.
Detecting cleaved fragments of known caspase substrates (PARP, lamin A, cytokeratin-18) via western blot and immunostaining.

Main Results:

Caspase activation can be reliably assessed through various biochemical and histological techniques.
Western blotting and immunostaining are effective for detecting cleaved caspases and their substrates.
Multiple methods provide complementary data for accurate caspase activity assessment.

Conclusions:

Confirming caspase activation in mouse tissues requires employing more than one detection method.
A combination of techniques ensures robust and accurate identification of apoptotic pathway execution.