Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

G Protein-coupled Receptors01:15

G Protein-coupled Receptors

14.1K
G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
14.1K
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

2.4K
2.4K
Transducer Mechanism: G Protein–Coupled Receptors01:30

Transducer Mechanism: G Protein–Coupled Receptors

9.0K
G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
GPCRs are also called heptahelical,...
9.0K
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

91.4K
G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
91.4K
G-protein Coupled Receptors01:21

G-protein Coupled Receptors

5.7K
5.7K
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

8.9K
Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
8.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural characterization of kappa-opioid receptor dimer in complex with two G proteins.

Nature communications·2026
Same author

Structural and Functional Basis of SSTR5 Homodimerization in Controlling Receptor Signaling and Pasireotide Response in Corticotroph Adenomas.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

EDNRB-dependent endothelin signaling reduces proliferation and promotes proneural-to-mesenchymal transition in gliomas.

Molecular oncology·2026
Same author

Divergent Roles of mGlu2 and mGlu3 Receptors in Amyloid-β Production and Cognitive Dysfunctions in Alzheimer's Disease.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Functional and structural basis of a negative allostery within GABA<sub>B</sub> hetero-tetramers.

Nature communications·2026
Same author

Allosteric activation of the glutamate receptor mGlu2 by the serotonin receptor 5-HT<sub>2A</sub>.

Nature communications·2026

Related Experiment Video

Updated: May 2, 2026

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
07:16

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Published on: August 16, 2018

14.1K

Complex GABAB receptor complexes: how to generate multiple functionally distinct units from a single receptor.

Chanjuan Xu1, Wenhua Zhang1, Philippe Rondard2

  • 1Cellular Signaling Laboratory, Key Laboratory of Molecular Biophysics of Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology Wuhan, China.

Frontiers in Pharmacology
|February 28, 2014
PubMed
Summary

Gamma-aminobutyric acid (GABA) B receptors, crucial for brain function and treating neurological disorders, exhibit surprising signaling complexity. This complexity arises from various molecular interactions, not from multiple receptor subtypes.

Keywords:
G-protein coupled receptor interacting proteinsGABAB receptordimerslarge oligomerssignal transduction

More Related Videos

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
10:59

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Published on: August 17, 2022

3.0K
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
07:30

HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

Published on: July 9, 2016

6.4K

Related Experiment Videos

Last Updated: May 2, 2026

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission
07:16

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Published on: August 16, 2018

14.1K
Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET
10:59

Visualizing the Conformational Dynamics of Membrane Receptors Using Single-Molecule FRET

Published on: August 17, 2022

3.0K
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice
07:30

HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

Published on: July 9, 2016

6.4K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Pharmacology

Background:

  • Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system.
  • GABA exerts its effects through GABA-A/C (ligand-gated) and GABA-B (G protein-coupled) receptors.
  • GABA-B receptors are critical for synaptic modulation and are therapeutic targets for brain diseases like addiction.

Purpose of the Study:

  • To investigate the signaling complexity of the GABA-B receptor.
  • To explore mechanisms that increase the functional diversity of the single identified GABA-B receptor type.

Main Methods:

  • Review of recent molecular and cellular studies on GABA-B receptor function.
  • Analysis of factors influencing GABA-B receptor signaling pathways.

Main Results:

  • Cloning revealed only two genes forming a single type of GABA-B receptor, contrary to expectations of multiple subtypes.
  • Signaling complexity is significantly increased through receptor stoichiometry, subunit isoforms, and interactions with other proteins and receptors.
  • Cell-surface expression and localization also contribute to functional diversity.

Conclusions:

  • The GABA-B receptor, despite being a single type, exhibits remarkable signaling complexity.
  • Mechanisms like subunit interactions and protein crosstalk enhance its functional repertoire.
  • These findings offer new insights into neuromodulation and potential therapeutic strategies for neurological conditions.