Direct detection of human herpesvirus 6B by the LAMP method using newly developed dry-reagents
Tetsushi Yoshikawa1, Takahiro Matsuo1, Yoshiki Kawamura1
1Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Journal of Virological Methods
|March 5, 2014
Abstract:
The reliability of the HHV-6B LAMP using the dry-reagent method was evaluated using serum samples obtained from febrile children. The sensitivity of the original and dry-reagent methods was 10 copies/reaction and 100 copies/reaction, respectively. The dry-reagent LAMP method was highly sensitive (94.0%) and specific (96.0%) for the detection of HHV-6B.
