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Reproductive Cloning

Reproductive Cloning

Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...

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Updated: May 2, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

High-throughput cloning for biophysical applications.

Keehwan Kwon¹, Scott N Peterson

¹J. Craig Venter Institute, 9704 Medical Center Dr., Rockville, MD, 20850, USA, kkwon@jcvi.org.

Methods in Molecular Biology (Clifton, N.J.)

|March 5, 2014

Summary

This summary is machine-generated.

High-throughput gene cloning is essential for proteomics. Ligation-independent cloning (LIC) offers a rapid, cost-effective, and automatable method, particularly for 96-well formats.

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Area of Science:

Molecular Biology
Structural Biology
Proteomics

Background:

High-throughput gene cloning is crucial for post-genomic functional and structural proteomics.
Ligation-independent cloning (LIC) is widely adopted by major structural biology centers.
The LIC method is valued for its ease of use, low cost, and speed.

Purpose of the Study:

To describe procedures for implementing ligation-independent cloning (LIC) in a 96-well format.
To facilitate automation in high-throughput cloning workflows.
To support large-scale structural biology initiatives.

Main Methods:

Adaptation of the ligation-independent cloning (LIC) technique.
Implementation in a 96-well microplate format.
Focus on automation compatibility.

Main Results:

Demonstration of LIC procedures suitable for 96-well formats.
Highlighting the adaptability of LIC for automation.
Establishing a foundation for high-throughput cloning pipelines.

Conclusions:

The LIC method is highly adaptable for high-throughput gene cloning in 96-well formats.
LIC facilitates automation, making it suitable for large-scale structural biology.
This approach streamlines critical steps in proteomics research.