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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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Related Experiment Video

Updated: May 2, 2026

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Published on: October 16, 2014

Eukaryotic expression systems for structural studies.

Christopher A Nelson¹, William H McCoy, Daved H Fremont

¹Center for Structural Genomics of Infectious Diseases, Computational Institute, University of Chicago, Chicago, IL, 60557, USA.

Methods in Molecular Biology (Clifton, N.J.)

|March 5, 2014

Summary

This summary is machine-generated.

This protocol details protein production in mammalian cells using transient transfection. This simple, successful method aids eukaryotic protein expression with minimal equipment, utilizing 6-HIS or Fc fusion tags for purification.

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Last Updated: May 2, 2026

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Published on: October 16, 2014

High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension

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Published on: December 28, 2015

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells

Published on: July 31, 2012

Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Mammalian cell expression systems are crucial for producing complex proteins.
Transient transfection offers a rapid method for eukaryotic protein production.

Purpose of the Study:

To describe a straightforward protocol for protein production in mammalian cells.
To highlight the utility of fusion tags for protein purification.

Main Methods:

Protein production via transient transfection in mammalian cells.
Utilizing expression constructs with 6-HIS or Fc fusion tags.
Recovery of expressed proteins using affinity chromatography.

Main Results:

The described method is one of the simplest available for eukaryotic protein expression.
The protocol requires minimal specialized equipment.
The method demonstrates a reasonably high success rate for protein production.

Conclusions:

Transient transfection in mammalian cells is an accessible and effective method for protein production.
The use of 6-HIS or Fc fusion tags simplifies protein recovery and purification.
This protocol is suitable for researchers needing to produce proteins in eukaryotic systems with limited resources.