Small molecule recognition of poly(A)
1Zentiva Health Products Co., Lüleburgaz, Kırklareli, Turkey.
Methods in Molecular Biology (Clifton, N.J.)
|March 5, 2014
Abstract:
The binding of small molecules to non-canonical nucleic acid structures has been a major focus of rational drug design. Among the non-canonical nucleic acid structures, targeting poly(A) using small molecules has attracted a special interest due to the cellular functions of poly(A) tails. Here, the methods for determining the binding of a small molecule to poly(A) using UV-visible(UV-Vis) and Circular Dichroism (CD) Spectroscopy are described. Experiments used in determining the melting temperature, binding stoichiometry and dissociation constant of poly(A)-small molecule systems are depicted.
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