[Detection of replication sites in the nuclei of plant cells using semithin sections]

A novel approach for the detection of replication sites in plant cells nuclei is described. Included nucleotide (EdU) was detected using "click"-chemistry in semithin sections of the material embedded in acrylic resin. The usage of the protocol introduced allows: 1) to preserve the intact morphology of cells, 2) to work with any tissue, and 3) to obtain high-resolution microscopy (especially, axial).