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Updated: May 2, 2026
Single Cell Collection of Trophoblast Cells in Peri-implantation Stage Human Embryos
Published on: June 12, 2020
Post-maturation zona perforation improves porcine parthenogenetic trophoblast culture
I M Saadeldin1, S J Kim2, Y B Choi2
1Department of Theriogenology and Biotechnology, College of Veterinary Medicine and The Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea; Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
Abstract:
This study was designed to optimize a method to improve porcine parthenogenetic embryo hatching and trophoblast culture. Mature oocytes (D0PPA) and day 6 blastocysts (D6PPA) were perforated with a 20 μm diameter needle for assisted hatching. The two groups showed a significant difference in hatching rate and blastocyst cell doubling when compared to a non-perforated control group. D0PPA blastocysts were able to form tertiary trophoblast colonies but D6PPA and control groups were not able to grow beyond primary colonies. Quantitative real-time PCR analysis showed significant differences in BAX, BAX/BCL2L1 and HSP70-2 mRNA expression between the experimental groups.
