Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasmids can stably transform yeast mitochondria lacking endogenous mtDNA.

T D Fox1, J C Sanford, T W McMullin

  • 1Section of Genetics and Development, Cornell University, Ithaca, NY 14853.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A possible heterotic threshold in the potato and its implications for breeding.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Transient expression of foreign genes in rice, wheat and soybean cells following particle bombardment.

Plant molecular biology·2013
Same author

An attempt to induce "egg transformation" in Lycopersicon esculentum Mill. using irradiated pollen.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Attempted "egg transformation" in Zea mays L., using irradiated pollen.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Further attempts to induce "egg transformation" using irradiated pollen.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

"Egg transformation" induced by irradiated pollen in Nicotiana: a re-examination.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Researchers created synthetic mitochondrial DNA (mtDNA) in yeast using bacterial plasmids. This novel approach allows for the study of mitochondrial gene function and mutations in yeast.

Area of Science:

  • Mitochondrial genetics
  • Yeast molecular biology
  • Synthetic biology

Background:

  • Mitochondrial DNA (mtDNA) is essential for cellular respiration.
  • Yeast strains lacking mtDNA (rho0) provide a model for studying mtDNA function.
  • Introducing foreign DNA into mitochondria is challenging.

Purpose of the Study:

  • To develop a method for transforming yeast mitochondria with a bacterial plasmid containing the oxi1 gene.
  • To investigate the behavior and genetic expression of plasmid-derived mtDNA sequences in yeast.
  • To create "synthetic rho-" yeast strains for studying mitochondrial gene function and mutations.

Main Methods:

  • High-velocity microprojectile bombardment to introduce a bacterial plasmid carrying the oxi1 gene into rho0 yeast.

Related Experiment Videos

  • Analysis of plasmid DNA behavior, including concatenation and reiteration, within yeast mitochondria.
  • Genetic manipulation and expression studies in diploid yeast strains, including marker rescue and trans-complementation experiments.

    • Main Results:

    • Plasmid DNA in transformants formed concatemers similar in size to wild-type mtDNA.
    • Plasmid-derived oxi1 sequences were stably maintained and genetically expressed in yeast mitochondria.
    • Functional expression of the plasmid-borne oxi1 gene was demonstrated through recombination and trans-complementation.

    Conclusions:

    • Bacterial plasmids can be successfully used to create functional "synthetic rho-" yeast strains.
    • This transformation method enables the study of in vitro-generated mutations in mitochondrial genes.
    • The developed system facilitates the examination of altered mitochondrial gene function in a controlled manner.