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Related Experiment Video

Updated: May 2, 2026

Mapping Infant Immunity with Minimal Input: Integrative Single-cell and Multiomic Profiling

Mapping Infant Immunity with Minimal Input: Integrative Single-cell and Multiomic Profiling

Published on: April 3, 2026

Profiling human antibody responses by integrated single-cell analysis.

Adebola O Ogunniyi¹, Brittany A Thomas¹, Timothy J Politano¹

¹Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, United States.

|March 8, 2014

Summary

This summary is machine-generated.

This study introduces a novel nanowell platform for detailed analysis of antigen-specific B cells and their antibodies. This technology aids in understanding immune responses and developing effective vaccines.

Keywords:

Humoral responses Immune profiling Microengraving Nanowells Plasma and memory B cells analysis

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Published on: August 22, 2019

Area of Science:

Immunology
Molecular Biology
Biotechnology

Background:

Characterizing antigen-specific B cells is crucial for discovering novel antibodies and understanding vaccine-induced humoral immunity.
Existing methods like flow cytometry and microtechnologies have limitations in comprehensive B cell profiling.

Purpose of the Study:

To present an integrated analytical platform using nanowells for detailed profiling of human B cells.
To demonstrate the platform's capability in analyzing immunophenotypes, antibody isotypes, antigen specificity, affinity, and B cell receptor genes.

Main Methods:

Utilizes arrays of subnanoliter wells (nanowells) for single-cell analysis.
Combines on-chip image cytometry, microengraving, and single-cell reverse transcription PCR (RT-PCR).

Main Results:

The platform successfully profiles human B cells, including immunophenotypes, antibody isotypes, and antigen specificity/affinity.
Identified antigen-specific neutralizing antibodies in HIV-infected subjects.
Demonstrated compatibility with plasmablasts, plasma cells, activated memory B cells, and limited cell numbers from tissue biopsies.

Conclusions:

The integrated nanowell platform provides comprehensive characterization of antigen-specific B cells.
This technology is well-suited for analyzing human humoral responses, evaluating vaccine efficacy, and studying B cells from various anatomical sites.