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Updated: May 2, 2026

Author Spotlight: Scaling Microalgal Biotechnology for Enhanced Biomethane Production
Published on: March 22, 2024
Bioconversion of algae to methane and subsequent utilization of digestate for algae cultivation: a closed loop
Sanjeev Kumar Prajapati1, Pushpendar Kumar2, Anushree Malik2
1Applied Microbiology Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology (IIT) Delhi, Hauz Khas, New Delhi 110016, India; Biogas Research Laboratory, Centre for Rural Development and Technology, Indian Institute of Technology (IIT) Delhi, Hauz Khas, New Delhi 110016, India.
Abstract:
The present investigation was targeted on anaerobic digestion of Chroococcus sp. and utilization of resultant "Liquid Digestate" for its further biomass production. The algal biomass has biomethane potential of 317.31 ± 1.9 mL CH4 g(-1) VSfed. Regular process monitoring revealed that process was stable throughout the experiments. The "Liquid Digestate" was explored as nutrient supplement for further algal growth. Diluted "Liquid Digestate" (30% concentration) was found optimal for algal growth (0.79 ± 0.064 g L(-1)). Simultaneously, 69.99-89.31% removal in nutrient and sCOD was also recorded with algal growth. Interestingly, higher growth was observed when rural sector wastewater (1.29 ± 0.067 g L(-1)) and BG11 broth (1.42 ± 0.102 g L(-1)) was used for diluting the "Liquid Digestate". The current findings have practically proven the feasibility of hypothesized "closed loop process".
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