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Updated: May 2, 2026

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Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
Published on: August 22, 2019
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Digital colour tone for fluorescence sensing: a direct comparison of intensity, ratiometric and hue based
Aron Hakonen1, Jonathon E Beves, Niklas Strömberg
1Department of Applied Physics, Chalmers University of Technology, Gothenburg, Sweden. hakonen@chalmers.se.
The Analyst
|March 8, 2014
Abstract:
The hue parameter of HSV colour-space for digital imaging is shown to be accessible for convenient quantitative fluorescence imaging. A commercially available pH probe was utilized in solution and incorporated into optical μ-sensors for microscopy applications.

