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Electrochemiluminescence aptasensor for adenosine triphosphate detection using host-guest recognition between
Hong Chen1, Qiong Chen1, Yingying Zhao1
1Department of Chemistry, State Key Laboratory of Precision Spectroscopy, East China Normal University, Shanghai 200241, PR China.
Talanta
|March 11, 2014
Summary
This study introduces a novel electrochemiluminescence aptasensor for detecting adenosine triphosphate (ATP). The sensor utilizes host-guest interactions for sensitive and label-free ATP quantification.
Area of Science:
- Analytical Chemistry
- Biosensors
- Nanotechnology
Background:
- Adenosine triphosphate (ATP) is a crucial biomarker.
- Developing sensitive and label-free detection methods for ATP is essential.
Purpose of the Study:
- To design a sensitive and label-free electrochemiluminescence (ECL) aptasensor for ATP detection.
- To utilize host-guest recognition for enhanced sensor performance.
Main Methods:
- Immobilization of an ATP-binding aptamer on a glassy carbon electrode (GCE).
- Host-guest recognition between a metallocyclodextrin complex [tris(bipyridine)ruthenium(II)-β-cyclodextrin] and the aptamer.
- Monitoring ECL signal changes upon ATP binding.
Main Results:
- A significant decrease in ECL intensity was observed in the presence of ATP due to restricted host-guest recognition.
- An excellent logarithmic linear relationship was achieved between ECL decrease and ATP concentration (0.05–10.0 nM).
- A low detection limit of 0.01 nM (S/N ratio of 3) was obtained.
Conclusions:
- The developed ECL aptasensor demonstrates high sensitivity and selectivity for ATP detection.
- The label-free approach avoids time-consuming signal-labeling procedures.
- This sensor presents a promising model for detecting aptamer-specific targets.
Keywords:
Adenosine triphosphateAptasensorElectrochemiluminescenceHost–guest recognitionMetallocyclodextrin
