[Isolation and study of polyadenylated RNA from Bacillus intermedius]

A poly(A)+RNA fraction was isolated from the overall RNA of Bacillus intermedius using chromatography on poly(U) Sepharose and was shown to be electrophoretically heterogeneous. The presence of a polyadenylate segment was confirmed by hybridization with polyuridine. The biological activity of the poly(A)+RNA was proved by the translation in Xenopus laevis oocytes. The dynamics of poly(A)+RNA synthesis was studied in the course of B. intermedius growth and the content of poly(A)+RNA was assayed in the cells grown in different media.