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Updated: May 2, 2026

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Palindromic sequence artifacts generated during next generation sequencing library preparation from historic and

Bastiaan Star1, Alexander J Nederbragt1, Marianne H S Hansen1

  • 1Centre for Ecological and Evolutionary Synthesis (CEES), Department of Biosciences, University of Oslo, Oslo, Norway.

Plos One
|March 11, 2014
PubMed
Summary

A novel DNA sequencing artifact, forming interrupted palindromes, has been identified in historic Atlantic cod samples. This artifact, caused by hairpin loops in single-stranded DNA, introduces sequence bias and affects DNA yield in ancient DNA studies.

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