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Updated: May 2, 2026
17:01
Acquiring Fluorescence Time-lapse Movies of Budding Yeast and Analyzing Single-cell Dynamics using GRAFTS
Published on: July 18, 2013
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Scoring and manipulating gene position and dynamics using FROS in budding yeast
Isabelle Loïodice1,2,3, Marion Dubarry3,4, Angela Taddei1,2,3
1Institut Curie, Centre de Recherche, Paris, France.
Current Protocols in Cell Biology
|March 11, 2014
Summary
Fluorescent repressor operator systems (FROS) enable chromatin dynamics studies in living cells. This review discusses FROS applications and limitations in budding yeast, a powerful model organism for genome organization research.
Area of Science:
- Cellular Biology
- Genomics
- Microscopy
Background:
- The spatial arrangement of the genome within the nucleus significantly influences its function.
- Studying chromatin dynamics in living cells is crucial for understanding genome regulation.
- Fluorescent repressor operator systems (FROS) have emerged as a key technology for this purpose.
Purpose of the Study:
- To review the applications of gene tagging with FROS for studying chromatin dynamics.
- To discuss the limitations and challenges associated with FROS in different species.
- To highlight the utility of FROS in budding yeast as a model organism.
Main Methods:
- Utilizing homologous recombination to integrate protein-binding sites at specific genomic loci.
- Employing fast microscopy to monitor the fluorescence of tagged DNA-binding proteins.
- Combining FROS with targeting assays and genetic analysis in living cells.
Main Results:
- FROS, particularly in budding yeast, has proven highly effective for investigating chromatin dynamics.
- The technique allows for real-time observation of genome organization and its functional implications.
- Recurrent issues and limitations of FROS across various species have been identified.
Conclusions:
- FROS is a powerful tool for dissecting the determinants and functions of chromatin dynamics in living cells.
- Budding yeast serves as an excellent model for advancing FROS applications in genome organization studies.
- Further research is needed to address the limitations of FROS for broader applicability.
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