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Updated: Aug 26, 2026

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
Published on: May 7, 2018
Salivary gland morphology after alpha-adrenergic and cholinergic stimulation. An ultrastructural study
1Department of Otolaryngology, Linköping University Hospital, Sweden.
Abstract:
The submandibular glands in 100 rats were subjected to different sialagogues: carbachol, clonidine, noradrenalin, and cyclocytidine. The morphological effects of these drugs were compared ultrastructurally. Clonidine, which is an alpha-2-agonist affected mostly the mucous cells, with formation of cytoplasmic vacuoles between the mucous droplets. Noradrenalin and cyclocytidine, both alpha-1-agonists, showed depletion of secretory granules in the serous cells. Noradrenalin also induced cytoplasmic disorganization with enlarged mitochondria, irregular endoplasmic reticulum, and shrinking of the secretory lumen. Carbachol, a cholinergic agonist, was found to produce widespread vacuolization in the cytoplasm of both serous and mucous cells. Small vesicles appeared and mitochondria were destroyed. To induce experimentally a complete depletion of granular serous cells, without other changes in ultrastructural morphology, cyclocytidine was found to be the most efficient drug.
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