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A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis
G J Wadsworth1, M G Redinbaugh, J G Scandalios
1Department of Genetics, North Carolina State University, Raleigh 27695-7614.
Analytical Biochemistry
|July 1, 1988
Abstract:
A small-scale method for the isolation of total RNA from plant tissue is described. The method provides RNA of suitable quantity and quality from 0.2 g fresh tissue for the detection of mRNA species by RNA blot analysis. The entire procedure is adapted to 1.5-ml microfuge tubes and takes less than 5 h. This method is well suited for the isolation of RNA from large numbers of samples or from samples of limited quantity.