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Methods for quantifying lysophosphatidic acid in body fluids: a review
Anna Jesionowska1, Elzbieta Cecerska1, Barbara Dolegowska1
1Department of Laboratory Diagnostics and Molecular Medicine, Pomeranian Medical University, 70-111 Szczecin, Poland.
Analytical Biochemistry
|March 12, 2014
Summary
Lysophosphatidic acid (LPA) is a bioactive lipid implicated in cell signaling. Elevated LPA in ovarian cancer patients suggests its use as a diagnostic marker, with various methods available for its quantification.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Background:
- Lysophosphatidic acid (LPA) is a bioactive lipid mediator.
- LPA participates in crucial physiological and pathological cellular processes.
- Elevated plasma LPA levels are a hallmark of epithelial ovarian cancer.
Purpose of the Study:
- To highlight the significance of LPA in cellular signaling and disease.
- To underscore the potential of LPA as a diagnostic marker for ovarian cancer.
- To review methodologies for LPA quantification.
Main Methods:
- Radioenzymatic assays
- Fluorometric assays
- Colorimetric assays
- Immunoenzymatic assays
- Capillary electrophoresis
- Gas chromatography
- Thin-layer chromatography
- Liquid chromatography
- MALDI-TOF mass spectrometry
Main Results:
- LPA levels are significantly increased in ovarian cancer patients.
- Various analytical techniques can quantify total LPA and individual LPA species.
- The choice of method depends on whether total LPA or specific molecular species require determination.
Conclusions:
- LPA is a relevant biomarker in epithelial ovarian cancer.
- Accurate quantification of LPA is achievable through diverse analytical approaches.
- Further research into LPA's role in cancer may yield novel diagnostic and therapeutic strategies.
