Microfluidic-based generation of size-controlled, biofunctionalized synthetic polymer microgels for cell
Devon M Headen1, Guillaume Aubry, Hang Lu
1Woodruff School of Mechanical Engineering, 315 Ferst Dr NW, Atlanta, GA, 30332, USA; Petit Institute for Bioengineering and Bioscience, 311 Ferst Dr NW, Atlanta, GA, 30332, USA.
Advanced Materials (Deerfield Beach, Fla.)
|March 12, 2014
Summary
Synthetic hydrogels create protective microenvironments for cell encapsulation. A microfluidic method engineers these microgels for controlled delivery, supporting high cell viability and function.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Microfluidics
Background:
- Synthetic hydrogels offer immunoprotection and support for encapsulated cells.
- Developing methods for precise control over microgel properties is crucial for cell delivery applications.
Purpose of the Study:
- To present a microfluidic strategy for generating biofunctionalized synthetic microgel particles.
- To achieve precise control over particle size and molecular permeability for cell and protein delivery.
Main Methods:
- Utilized a microfluidic approach to synthesize biofunctionalized hydrogel microparticles.
- Engineered microgels with controlled size and tunable molecular permeability.
- Encapsulated human stem cells and islets within the engineered microcapsules.
Main Results:
- Demonstrated precise control over microgel particle size and molecular permeability.
- Achieved high encapsulation efficiency for cells and proteins.
- Confirmed sustained high cell viability and function of encapsulated human stem cells and islets.
Conclusions:
- The developed microfluidic strategy enables the creation of advanced microcapsules for cell and protein delivery.
- Engineered synthetic microgels provide a supportive and immunoprotective microenvironment.
- This technology holds promise for regenerative medicine and therapeutic applications.
